Pureflow Bio

PUREFLOW BIO / ENGINEERING FIELD GUIDE

Carbon Dioxide in RASThe Silent Stress and How to Strip It

Why CO2 builds up in recirculating systems, what levels cause stress, and how aeration, degassing and trickle filters remove it.

6 MIN GUIDEUPDATED NOVEMBER 2026
Reference illustration for Carbon Dioxide in RAS: The Silent Stress and How to Strip ItEQUIPMENT REFERENCE
Equipment reference illustration. Confirm the supplied model configuration and installation arrangement from its approved documentation.
01Understand the mechanismInspect the relevant process stages.
02Review the operating dutyKeep assumptions and measured values visible.
03Prepare the next decisionUse the guide, tools and project checks.
INTERACTIVE PROCESS ATLAS

See the mechanism.
Understand the duty.

Select a route or a stage to inspect the process. The cutaway explains the equipment and water environment; it is a teaching illustration, not a project drawing.

Swipe horizontally to inspect the equipment. Stage buttons below also centre the selected component.
Sampling and biological nitrogen conversionPUREFLOW / INTERACTIVE PROCESS ATLASCONCEPT LAYOUT · NOT TO SCALETAN-NpH°CSAMPLE THE SYSTEMBIOLOGICAL CONVERSIONSTATE THE TEST BASISNH₄⁺ ⇄ NH₃ · NO₂⁻ · NO₃⁻CONCENTRATION, LOAD, CHEMICAL FORM AND SAFE LIMITS ARE DIFFERENT QUESTIONSMotion explains the process; it is not a measured flow rate or biological time model.
A useful sample record includes the method, unit, time, location and relevant water conditions.
Selected process stage

Measure the system

Record the test method, units and sampling location.

How it works

Use appropriate tests and record units, location, time, pH and temperature with the result.

What to establish
  • Record the test method, units and sampling location.
  • Keep concentrations, loads and chemical forms distinct.
  • Interpret results with species, pH, temperature and salinity.

Continue this guide ↗

Motion explains direction and mechanism; it does not represent measured performance. Geometry, stage order, pipe routes and equipment configuration are conceptual. Use the approved project design, operating instructions and verified model data.

Key takeaways

  • Why it builds up: Fish respiration and bacterial activity both produce CO2.
  • Levels to watch: Many fish and shrimp do well with CO2 kept around 15 to 20 mg/L or lower, and lethargy can show above about 50 mg/L.
  • How to remove it: Expose water to air.
  • The oxygen link: High CO2 makes low oxygen more dangerous because fish struggle to use what is there.
Reference illustration for Carbon Dioxide in RAS: The Silent Stress and How to Strip It
Equipment reference visual; not evidence of a completed installation.
THE SHORT ANSWER

Why it builds up

Fish respiration and bacterial activity both produce CO2. Dense stocking and low turbulence make it worse. If water just sits and circulates, carbon dioxide stays dissolved.

Carbon Dioxide in RAS - Pureflow Bio
Reference visual. Equipment configuration and project details must be confirmed separately.
WATER QUALITY

Levels to watch

Many fish and shrimp do well with CO2 kept around 15 to 20 mg/L or lower, and lethargy can show above about 50 mg/L. Sensitive species want less.

PLANNING WORKBENCH

Work with your own numbers.

Use the tools below to explore the relevant arithmetic. Enter measured values or declared assumptions. An estimate does not establish equipment capacity, biological performance or a safe operating limit.

Ideal dilution calculation

Estimate a single mixed water replacement from measured starting, replacement-water and target concentrations.

Assumptions and limits

A simple mass balance, not a safe operating target. Assumes complete mixing and no ongoing production or reaction. Species, chemistry and source-water suitability require review.

—Ideal replacement share · %
—Replacement volume · m³
Enter your values, or load the labelled arithmetic example. No design values are pre-filled.
FormulaReplacement fraction = (starting − target) ÷ (starting − replacement).
Convert units before you calculate
Choose units and enter a value.

Planning arithmetic · inputs remain visible for review · example values are not operating recommendations

WATER QUALITY

How to remove it

Expose water to air. Degassing towers, trickle filters and strong aeration all strip carbon dioxide. See trickle filters.

WATER QUALITY

The oxygen link

High CO2 makes low oxygen more dangerous because fish struggle to use what is there. Keep both in check. See dissolved oxygen.

WATER QUALITY

Signs

Slow growth, low pH that keeps falling, and fish that seem lethargic despite good oxygen.

PROJECT REVIEW

Make the measurements comparable

Use these questions to prepare a project brief or an operating review. Check an item only when the information has been recorded for your installation.

0 of 3 checked

This checklist is a working aid in the current page session, not a saved maintenance record.

QUESTIONS

Carbon Dioxide in RAS FAQ

How is CO2 measured?

With a titration test kit or a probe made for it. Calculating it from pH and alkalinity is also common.

Do ponds have a CO2 problem?

Usually less, because water exposes more surface to air, but dense stocking in tanks can.

KEEP READING

Discuss your system with Pureflow.

Share your application, operating volume, actual flow, loading and the question you are trying to resolve.

Discuss on WhatsApp ↗
Scroll to Top